Journal: RSC Advances

Article Title: Mononuclear Mg( ii ) and Ca( ii ) metallocarboxylates of N -phthaloyl- γ -aminobutyric acid: synthesis, characterization, ds-DNA binding, preliminary anticancer evaluation against MDA-MB-231 cells, and computational studies

doi: 10.1039/d6ra07684j

Figure Lengend Snippet: Anticancer activity of NY0, NY1 and NY2 against triple-negative MDA-MB-231 breast cancer cell lines: (a) cytotoxic effect against MDA-MB-231 cell lines and (b) viability against normal skin HaCaT cells.

Article Snippet: The triple-negative breast cancer (TNBC) cell line MDA-MB-231 (ATCC HTB-26) and the normal human breast epithelial cell line MCF-10A (ATCC CRL-10317) used in this study were purchased from the American Type Culture Collection (ATCC), USA.

Techniques: Activity Assay

Journal: RSC Advances

Article Title: Mononuclear Mg( ii ) and Ca( ii ) metallocarboxylates of N -phthaloyl- γ -aminobutyric acid: synthesis, characterization, ds-DNA binding, preliminary anticancer evaluation against MDA-MB-231 cells, and computational studies

doi: 10.1039/d6ra07684j

Figure Lengend Snippet: IC 50 values for the treated MDA-MB-231 cancer and MCF-10A normal cells

Article Snippet: The triple-negative breast cancer (TNBC) cell line MDA-MB-231 (ATCC HTB-26) and the normal human breast epithelial cell line MCF-10A (ATCC CRL-10317) used in this study were purchased from the American Type Culture Collection (ATCC), USA.

Techniques:

Journal: International Journal of Nanomedicine

Article Title: Folic Acid-Conjugated Albumin-Stabilized Gold–Terbium Nanoparticles for SPCCT/MRI Imaging: An in vitro Cellular and Phantom Study

doi: 10.2147/IJN.S584189

Figure Lengend Snippet: Schematic illustration of synthesis of BSA@Au/Tb-FA nanoparticle, biological and multimodal imaging evaluation, functional roles, and proposed cellular uptake pathway in MDA-MB-231 cells.

Article Snippet: Triple-negative breast cancer MDA-MB-231 cells (American Type Culture Collection, ATCC) were used to assess NP cytotoxicity using an ATP-based luminescent cell viability assay (CellTiter-Glo , Promega Corporation, USA).

Techniques: Imaging, Functional Assay

Journal: International Journal of Nanomedicine

Article Title: Folic Acid-Conjugated Albumin-Stabilized Gold–Terbium Nanoparticles for SPCCT/MRI Imaging: An in vitro Cellular and Phantom Study

doi: 10.2147/IJN.S584189

Figure Lengend Snippet: ( a ) CellTiter-Glo–based in vitro viability assay of MDA-MB-231 cells following 24 h exposure to BSA@Au/Tb NPs and BSA@Au/Tb-FA NPs. ( b ) Corresponding IC 50 values, determined to be ~397 μg/mL for BSA@Au/Tb NPs and ~352 μg/mL for BSA@Au/Tb-FA NPs. The fitted curves showed strong correlations with R 2 values of 0.9542 and 0.9600, respectively.

Article Snippet: Triple-negative breast cancer MDA-MB-231 cells (American Type Culture Collection, ATCC) were used to assess NP cytotoxicity using an ATP-based luminescent cell viability assay (CellTiter-Glo , Promega Corporation, USA).

Techniques: In Vitro, Viability Assay

Journal: International Journal of Nanomedicine

Article Title: Folic Acid-Conjugated Albumin-Stabilized Gold–Terbium Nanoparticles for SPCCT/MRI Imaging: An in vitro Cellular and Phantom Study

doi: 10.2147/IJN.S584189

Figure Lengend Snippet: Flow-cytometry analysis of cellular uptake of BSA@Au/Tb and BSA@Au/Tb-FA nanoparticles in MDA-MB-231 cells. ( a ) FSC-A/SSC-A gating of the main cell population. ( b ) Singlet-cell gating using SSC-A versus SSC-H. ( c ) Unstained control used to establish background fluorescence. ( d ) Representative fluorescence histograms showing a concentration-dependent rightward shift after treatment with FA-BSA@Au/Tb nanoparticles. ( e ) Concentration-dependent Mean fluorescence intensity (MFI) for BSA@Au/Tb and FA-BSA@Au/Tb at 25, 50, and 100 µg/mL.( f ) Direct comparison of MFI between the two formulations at each concentration. Data are presented as mean ± SD from technical replicates within a single experimental run. Statistical comparisons were performed using one-way ANOVA followed by Tukey’s post hoc test. n.s., not statistically significant; *** p ≤ 0.001.

Article Snippet: Triple-negative breast cancer MDA-MB-231 cells (American Type Culture Collection, ATCC) were used to assess NP cytotoxicity using an ATP-based luminescent cell viability assay (CellTiter-Glo , Promega Corporation, USA).

Techniques: Flow Cytometry, Analysis, Control, Fluorescence, Concentration Assay, Comparison

Journal: International Journal of Nanomedicine

Article Title: Folic Acid-Conjugated Albumin-Stabilized Gold–Terbium Nanoparticles for SPCCT/MRI Imaging: An in vitro Cellular and Phantom Study

doi: 10.2147/IJN.S584189

Figure Lengend Snippet: Schematic illustration of synthesis of BSA@Au/Tb-FA nanoparticle, biological and multimodal imaging evaluation, functional roles, and proposed cellular uptake pathway in MDA-MB-231 cells.

Article Snippet: MDA-MB-231 cells were obtained from Cytion (catalog no. 300275).

Techniques: Imaging, Functional Assay

Journal: International Journal of Nanomedicine

Article Title: Folic Acid-Conjugated Albumin-Stabilized Gold–Terbium Nanoparticles for SPCCT/MRI Imaging: An in vitro Cellular and Phantom Study

doi: 10.2147/IJN.S584189

Figure Lengend Snippet: ( a ) CellTiter-Glo–based in vitro viability assay of MDA-MB-231 cells following 24 h exposure to BSA@Au/Tb NPs and BSA@Au/Tb-FA NPs. ( b ) Corresponding IC 50 values, determined to be ~397 μg/mL for BSA@Au/Tb NPs and ~352 μg/mL for BSA@Au/Tb-FA NPs. The fitted curves showed strong correlations with R 2 values of 0.9542 and 0.9600, respectively.

Article Snippet: MDA-MB-231 cells were obtained from Cytion (catalog no. 300275).

Techniques: In Vitro, Viability Assay

Journal: International Journal of Nanomedicine

Article Title: Folic Acid-Conjugated Albumin-Stabilized Gold–Terbium Nanoparticles for SPCCT/MRI Imaging: An in vitro Cellular and Phantom Study

doi: 10.2147/IJN.S584189

Figure Lengend Snippet: Flow-cytometry analysis of cellular uptake of BSA@Au/Tb and BSA@Au/Tb-FA nanoparticles in MDA-MB-231 cells. ( a ) FSC-A/SSC-A gating of the main cell population. ( b ) Singlet-cell gating using SSC-A versus SSC-H. ( c ) Unstained control used to establish background fluorescence. ( d ) Representative fluorescence histograms showing a concentration-dependent rightward shift after treatment with FA-BSA@Au/Tb nanoparticles. ( e ) Concentration-dependent Mean fluorescence intensity (MFI) for BSA@Au/Tb and FA-BSA@Au/Tb at 25, 50, and 100 µg/mL.( f ) Direct comparison of MFI between the two formulations at each concentration. Data are presented as mean ± SD from technical replicates within a single experimental run. Statistical comparisons were performed using one-way ANOVA followed by Tukey’s post hoc test. n.s., not statistically significant; *** p ≤ 0.001.

Article Snippet: MDA-MB-231 cells were obtained from Cytion (catalog no. 300275).

Techniques: Flow Cytometry, Analysis, Control, Fluorescence, Concentration Assay, Comparison